Journal: iScience
Article Title: Interferon-α and thymosin-α1 plus tislelizumab enhance CD8 + T cell cytotoxicity toward pancreatic ductal adenocarcinoma
doi: 10.1016/j.isci.2025.113053
Figure Lengend Snippet: Influence of tislelizumab, IFN-α, and Tα1 on the surface PD-1 expression of CD8 + T cells (A) Statistical analysis of the number of CD8 + T cells at different times within 14 days after incubation with 1 μg/mL of tislelizumab with or without IFN-α and Tα1 treatment, as determined by a CCK8 assay. (B–D) Statistical analysis of the levels of the secreted cytokines IL-2 (B), TNF-α (C), and IFN-γ (D) in suspensions of cultured CD8 + T cells after incubation with 1 μg/mL of tislelizumab with or without the addition of IFN-α and Tα1 for 14 days, as assayed by ELISA. The data were analyzed by one-way ANOVA and are presented as the means ± SDs of four or five biological replicates. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.
Article Snippet: IL-2 ELISA Kit , Sino Biological , Cat# KIT11848.
Techniques: Expressing, Incubation, CCK-8 Assay, Cell Culture, Enzyme-linked Immunosorbent Assay